Molecular diagnosis of intestinal protozoa in young adults and their pets in Colombia, South America

Intestinal parasitic infections have been considered a relevant public health problem due to the increased incidence worldwide. In developing countries, diarrhea and gastrointestinal symptoms cause impaired work capacity in adults and delayed rate growth in children. Enteric infections of unknown etiology can often lead to misdiagnosis, increased transmission, and morbidity. The aim of this study was to determine the prevalence of intestinal parasites in a young adult population and their pets. Stool samples from 139 university students and 44 companion animals were subjected to microscopy diagnosis using wet mounts, concentration by zinc sulphate flotation and staining techniques (Kinyoun and trichrome stain). Molecular diagnosis of protozoa was also performed by conventional PCR. The mean age was 24 years, 54% individuals were female, 46% were men, and 66% had at least one pet. The overall prevalence for at least one parasite was 74.8% and the rate of polyparasitism was 37.5%. Eighty-three patients (59.7%) were positive for Blastocystis spp., followed by Cryptosporidium spp. 24.5%, Endolimax nana 13.6%, Entamoeba dispar/E. moshkovskii 7.8% and Giardia intestinalis 1.4%. Molecular diagnosis substantially improved Cryptosporidium spp. and Blastocystis spp. detection and allowed to distinguish E. histolytica from commensals in the Entamoeba complex. Student’s pets were also examined for parasitism. Samples from 27 dogs, 15 cats, one rabbit and one hen were analyzed, and parasites were detected in 30 (68.2%) as follows: Cryptosporidium spp. (24) Giardia spp. (4), hookworm (3), Endolimax nana (2) and Toxoplasma gondii (1). Overall, university students showed high prevalence of parasitism and polyparasitism suggesting exposure to parasite infected animals and contaminated environments. Cryptosporidium spp. was the predominant pathogen in human and domestic animals, and it was only detected by PCR, pointing out the need for sensitive tests in diagnosis and surveillance. Control strategies to prevent the effects of parasitic infections in young population should consider pets as reservoirs and transmission source.

Introduction amplification methods has shown increased sensitivity and specificity which also offer multiplex formats and make feasible further genotype analysis [19,20].
In developing countries, better surveillance and improved routine diagnosis tests are required to understand the clinical effects of parasite infections in all affected populations and to reduce transmission. The aim of this study was to determine the prevalence of intestinal parasites in a student young adult population using conventional microscopy and molecular diagnosis of relevant protozoa. We also aimed to explore the factors that may favor the parasite spread and particularly, the potential role of animal exposure.

Ethics statement
The study protocol was approved by the Universidad del Valle Ethics Committee (Approval No.: 031/CNES/2010). Prior to the study, written informed consent from all participants was obtained.

Population study
A cross sectional study was conducted in a higher education institution, a public and research university in Cali, Colombia. Student population is predominantly from low and low-middle income backgrounds and comes from Cali and the nearest cities from the Departments of Valle del Cauca, Cauca, and Nariño. Cali is the third most populous city in southwest Colombia (3˚27 0 00 00 N, 76˚32 0 00 00 W) with dry-summer tropical climate and average annual precipitation between 900 to 1,800 mm and 25˚C temperature. A total of 139 students were enrolled in the study from August 2021 to January 2022. Students were recruited according with the following criteria: current enrollment at the university, age 18-40 and no parasite treatment for at least 6 months prior to the study. After an introductory talk to the research, the participants signed the consent form and then filled a self-administered questionnaire with socio-demographic, exposure, and clinical variables such as: gastrointestinal symptoms at the time of sampling, past or current health conditions, medications and current or previous parasite infections. All the data were included for analysis.
Fecal samples were obtained and processed using conventional microscopy-based techniques and then preserved in Shaudinn´s fixative for trichrome stain. The remainder of each sample was kept at -80˚C for further molecular analysis of enteric protozoa.
Additionally, fecal smears were prepared and subjected to modified acid -fast stain (Kinyoun) for detection of coccidian parasites [21]. For a better differentiation of parasite structures and quality control of microscopy, trichrome stains were also performed. Briefly, fecal smears were prepared and transferred for three minutes into D'Antoni iodine mixed with 70% ethanol. Then, the slides were placed in 70% ethanol for three minutes before being stained in the trichrome working solution (26 g/L) for 12 minutes and rinsed in 90% and 95% ethanol. Finally, the smears were placed in two changes of carbol-xylol solution and mounted using Permount Montage Medium (Fisher Chemical, TM) [21]. The smears were examined under a research microscope, Zeiss Axio imager A2 with an image analysis software (Zen Lite version 3.1).
Molecular assays DNA extraction. Stool samples were pretreated to improve DNA extraction. Briefly, 1 g of fecal sample was washed in sterile water and suspended in 250 μL of lysis buffer (0.15 M NaCl, 0.1 M EDTA, 0.5% sodium dodecyl sulphate, SDS) and vortexed for 20 minutes before being frozen at -80˚C overnight. Then, the samples were thawed and heated at 95˚C x 10 minutes before adding 3 μL of proteinase K (22 mg/mL) and then incubated for 10 minutes at 56˚C [22]. The homogenized samples were subjected to total DNA extraction using a fecal DNA extraction kit (IBI Scientific) according to the manufacturer´s recommendations.
PCR amplification. A conventional monoplex PCR was performed using specific primers to target genes from the pathogen protozoa E. histolytica, G. intestinalis, Cryptosporidium spp. [23] and D. fragilis [24] and for the commensal Blastocystis spp. [25]. PCR for Entamoeba dispar [26] (Table 1)  . Potential inhibition of PCR reactions was tested by amplification of known DNA in negative samples. PCR products were visualized in agarose gels using a Gel Doc system (LED FastGene FAS-DIGI PRO, NIPPON Genetics Europe).
Selected PCR amplified products were subjected to Sanger sequencing using an ABI 3730XL sequencer (Macrogen 1 Corp., Seoul, South Korea). The sequences were processed using Bioedit v 7.2.5 before alignment in CLUSTALW (MEGA Xv10.2.6). The BLAST tool (http://blast.ncbi.nlm.nih.gov/Blast.cgi) was used to determine the homology among sequences deposited in the National Center for Biotechnology Information (NCBI).

Statistical analysis
The data were collected in an Excel database (Microsoft office Excel 365, version 2204) and exported to the Statistical Package for the Social Sciences (SPSS, version 27) for analysis. Data were also computed using Epi info v 7.2.5. Univariate analysis was performed by frequency tables and means. Associations among demographic or behavioral variables and parasite infection were assessed using contingence tables and odds ratios were calculated with a 95% confidence interval. Chi square or Fisher´s exact tests were used for significance level, p values <0.05 were considered significant. Diagnostic techniques were compared using the Cohen´s kappa coefficient (κ) taking as reference the combined results of all techniques for each parasite. Interpretation of kappa values was as follows: < 0.20 none to slight, 0.21-0.40 fair, 0.41-0.60 moderate, 0.61-0.80 substantial, and 0.81-1.00 almost total agreement [27]. Multivariate logistic regression modeling was also used to identify the socio-demographic and behavioral variables associated with parasite infection. Over 30 variables in the univariate analysis, including those recognized as risk factors for parasite infection, were entered into a backward stepwise (Likelihood ratio) logistic regression model in SPSS v 27.

Socio-demographic characteristics of the study population
A total of 139 university students were included, 75 (54%) were female and 64 (46%) were male (male/female ratio 0.85) the mean age was 24 years old. Most of the study population (89, 64%) was among 18 and 24 years and coming from families considered in the low or middle low-income range. Most participants reported habits such as tap water drinking (115, 82.7%), fruit and vegetables consumption (122, 87.8% and 109, 78.4% respectively) and relevant animal

Parasite detection by microscopy
Seventy-one (51.1%) samples were formed stools, 62/139 (44.6%) were semiformed and 2/139 (1.4%) were watery, no bloody fecal samples were observed. Mucus was observed in two samples and leucocytes were not seen. Intestinal parasites were found in 63/139 (45.3%) students using microscopy techniques: 41/139 (29.5%) were positive for direct wet mount whereas 54/128 (42.2%) were seen by  (Table 3). From the negative samples for wet mount, which is the routine diagnosis test, one was detected by flotation concentration and 21 in trichrome stain. Results from the flotation technique were like those from direct wet mount except for the commensals Blastocystis spp. and Chilomastix mesnili which were not recovered using this method.
Overall, the presumptive frequency of pathogenic protozoa by microscopy was 8.6% (12/ 139). Protozoan loads were mostly low (54.2%), commensal parasites such as E. nana and E. coli showed higher cysts loads (33% and 50% respectively) whereas Blastocystis fecal loads were low (61.5%).  Table 3. Performance of each technique by parasite indicates that PCR was more effective for Blastocystis spp. and Cryptosporidium spp. than microscopy, with an overall agreement of 61.2% (Cohen´s k = 0.24, 95% CI: 0.097-0.369, fair agreement). In total, 83 stool samples were positive for Blastocystis spp. PCR positive samples were 49.6% (69) whereas 18.7% (26) were positive by wet mount and 33.8% (47) by any microscopy method. As shown in Fig 3, thirty-eight samples were positive by both PCR and microscopy, 31 were only positive by PCR and 14 were detected by microscopy using trichrome stain (69% agreement, Cohen´s k = 0.38, 95% CI: 0.232-0.528, fair agreement). For Cryptosporidium spp. all 34 cases were detected only by PCR (24.5% vs 0% for microscopy) with 0% positive agreement. However, the only two positive samples of G. intestinalis and one presumptive D. fragilis could not be confirmed by PCR. When comparing both techniques to identify the presence of any parasite in the samples, the prevalence estimated by conventional PCR was 65.5% (91) in contrast to 45.3% (63) with any microscopy technique.
Thirteen out of 34 positive samples, including the Cryptosporidium spp. and E. dispar DNA amplicons were subjected to Sanger sequencing. BLAST analyses showed >96% homology of Cryptosporidium amplicons under accession numbers ON668107 to ON668114 to the available GenBank sequences. One was identified as C. parvum and one as C. felis. The E. dispar

Single and multiple parasite infections
The overall parasite prevalence, which was determined by combining results from both microscopy and PCR techniques, was 74 (Table 3 and Fig 2). For specific parasite associations, it was seen that infection by Blastocystis spp. was frequently found in participants reporting fruit consumption (p = 0.0018, 95% CI 1.72-9.56) in contrast, taking home-prepared meals showed a protective effect (Fisher´s p = 0.038, 95% CI 0.07-0.93). No demographic or clinical factors were associated with Cryptosporidium spp. infections.

Animal exposure
Participants with pets were asked to bring samples from their companion animals; a total of 31 out of 92 (33.7%) owners submitted fecal pet samples. Forty-four samples were subjected to microscopy detection of parasites using the same combined methodology as for the owners. PCR for Cryptosporidium spp. was also performed. Stools from 27 dogs, 15 cats, one rabbit and one hen were examined. Thirty (68.2%) pets were positive for intestinal parasites, the remaining pets from 11 owners were negative. Cats showed more parasite infections than dogs, Cryptosporidium spp. was the most prevalent parasite in both species (8/15, 53.3% in cats, 14/27, 51.9% in dogs) followed by hookworms in dogs and Giardia spp. in cats (Table 4).
Human pathogens were also found in pets including one cat with Toxoplasma gondii infection. The pets with parasitic infections (30) belonged to 20 owners, the parasites found in pets and the parasitic status of owners are shown in Table 5. Seven out of 20 (35%) owners shared the same parasite type with their pets and 8 out of 20 (40%) owned more than one pet. Remarkably, it was seen that several pets in a household usually had the same parasite type (Table 5).

Discussion
Despite all the control measurements, intestinal parasites infections remain high worldwide. Considerable amount of research has focused on the most vulnerable populations but only few studies have explored the magnitude of parasite infections and associated factors in young adult population. The present work is the first study using combined microscopy and molecular diagnosis and exploring the animal exposure in this population. This survey revealed that 74.8% of the student young adults were infected with at least one parasite. Parasite infections were associated with those beneficiaries of the social assistance program (SISBEN), a Colombian system to identify vulnerable population in need. Here, the prevalence of parasite infection was higher when compared to previous studies in student young adults from several lowmiddle income countries ranging from 9% to 45.6% (Ethiopia 45.6% [28], Nigeria 9.3% [29], Bangladesh (23.1%) [30] and Iran 11.9% [31]) Table 6. Furthermore, two studies in Colombia have also shown high parasite prevalence in a similar population (81-83.4%) [32,33]. No associations were found in gender, age or participant´s procedence, this is in contrast with studies in Ethiopia where parasites were more frequently found in males [28], rural residents, married students and those enrolled longer than one year [34]. The majority of studies conducted in young adults has been performed using only conventional microscopy except those in Mexican students focused on molecular diagnosis and typing of Blastocystis spp. [15,35]. . Although this variability may be influenced by geographical area and the detection methods used, the university students seem to be also highly affected by parasites, moreover this population has the highest parasite prevalence when compared to similar studies worldwide [28-31, 34, 43]. Overall, ten different types of protozoa were found in the student´s fecal samples whereas geohelminths were not found.
Intestinal protozoans were also more prevalent in young adult populations from Ethiopia, Nigeria, Iran and Gaza [28,31,34,43], these cases have been attributed to environmental contamination, for instance in Nigeria parasite infections were associated with feeding habits and toilet type [29]. This agrees with previous studies in Colombia in university students and other populations [32,33,42] where prevailing parasite infections may be linked to the ingestion of food and water contaminated with protozoa [44]. A recent review has reported a high rate of contamination in unwashed vegetables and fruits ranging from 3 to 49% [45]. Although less frequent, STH such as A. lumbricoides, T. trichiura and hookworm have been found in young adults (Table 6), however these infections are more commonly found in pre-school and school aged children from endemic areas. In this work, helminths were assessed by three microscopy methods, but they were not detected. Overall, it has been suggested that conventional methods might underestimate the true prevalence of STH infections.
In this study, both microscopy-based techniques and PCR for protozoa were used for parasite detection. Similar to previous studies, we found low level of concordance between both conventional microscopy and PCR, as judged by the kappa index [37]. Comparison of several microscopy techniques showed that trichrome stain was able to detect most parasites, whereas zinc sulphate flotation technique was slightly better than the wet mount, and it was not suitable for Blastocystis spp. as the chemical seems to induce damage of the parasite´s membranes [46,47]. The combination of microscopy techniques used in this study presumptively detected 8% of pathogenic protozoa and no detection of Cryptosporidium spp. was reported using a modified acid-fast staining. However, PCR substantially improved parasite diagnosis by detecting Cryptosporidium spp. and Blastocystis spp. and allowing to differentiate E. histolytica from E. dispar and E. moshkovskii in the Entamoeba complex. Nevertheless, the only two cases of G. intestinalis reported by microscopy could not been confirmed by PCR, this may be due to several reasons such as sample inhibitors or incomplete cyst rupture. In this work, the most frequent pathogen was Cryptosporidium spp., and the most frequent commensal was Blastocystis spp. followed by E. nana. Comparison with other studies in young adults was limited by the detection methods (the others mainly based on microscopy) however, there was an agreement on the finding of Blastocystis spp., E. nana and Entamoeba complex [32] with exception of those in African countries where Blastocystis spp. was not reported [28,29,34]. For instance, in Ethiopia, the most frequent parasites reported in university students were Entamoeba complex (19.7-20.3%) and G. intestinalis (8.2-9.3%). Also, one study in Gaza mainly found Entamoeba complex (7.5%) and G. intestinalis (4.9%), in contrast with a work conducted in Bangladesh reporting A. lumbricoides (11.1%) followed by Entamoeba complex (4.9%) and G intestinalis (3.7%). In another study in Iran the prevailing parasites were Blastocystis spp. (4.5%) and G. intestinalis (3.5%). Previous studies in Colombia agreed with a high frequency of E. nana (44-78.8%) followed by Blastocystis spp. (61.9%) or E. coli (33%) [32,33]. Only one

PLOS ONE
study from Iran included microscopy testing for Cryptosporidium sp. but cases were not found [31].
Blastocystis infections are highly prevalent globally, with frequencies up to 100% in some regions [48,49]. In this survey, 59.7% of students were found infected with Blastocystis spp. which agrees with an earlier study in Colombia showing a colonization rate in students of 61.9% [32] and studies by Perez et al. and Guangorena et al. in Mexican students where Blastocystis spp. prevalence was 47% and 53% respectively, using microscopy and molecular approaches [15,35]. Although Blastocystis spp. is considered a colonizer it may have a role in modification of gut microbiota and subtype specific effects on the individuals. It has been suggested that dysbiosis of gastrointestinal microbiota may also lead to chronic infections due to Blastocystis spp. but its impact on health is still matter of study [35]. Blastocystis spp. is also a marker of environmental contamination, and transmission has been associated with contaminated food or water and animal reservoirs, particularly the cattle [16]. Here, Blastocystis spp. infection was associated with fruit consumption, which is in agreement with findings by Ospina et al. where this parasite was associated with consumption of fruits, juices and salads [32]. Although most participants (92%) reported fruit and vegetable washing, judged by the results, this practice was not performed regularly, or contaminated water was used to wash the produce.
The second most prevalent parasite and the main pathogen found in this study was Cryptosporidium spp., a well-known pathogen found in surface water and fresh vegetables, is a main etiological agent of foodborne and waterborne outbreaks worldwide [44]. Cryptosporidium spp. is a highly infectious parasite with a low infection dose of 10 or even less than 10 oocysts and representing high risk for consumers [50,51]. The main transmission route is drinking of untreated or contaminated water but various infection routes have been identified including livestock and person to person contact, foodborne transmission and contact with pets [52]. Outbreaks have been also reported in young veterinary students which are considered a highrisk population [53,54]. In this work, most participants reported drinking regular tap water and 66.2% owned at least one pet. Interestingly, 24% of the participants were positive for Cryptosporidium spp. and all the cases were detected only by PCR. This is in contrast with studies performed in young adults in Iran [31] and vulnerable groups in Colombia, Brazil and Venezuela where Cryptosporidium spp. was not detected [42,55,56]. Cryptosporidium prevalence in this survey was substantially higher compared with the prevalence estimated for the country (7.8%) and studies using PCR in several bioregions in Colombia and Cuba which reported infection rates ranging from 0% to 10.5% [57,58] but similar to one study in Colombia by Bryan et al. reporting 19.4% in an urban community [38]. Cryptosporidium spp. has been found in school children (2.4% to 9.8%) from Colombia with reports of C. hominis and C. meleagridis [37,59,60]. Globally, the estimated prevalence of Cryptosporidium sp. was 7.6% (95% CI 6.9-8.5), with the highest estimated prevalence of 69,6% in Mexico [11]. Nevertheless, these data can be still substantially underestimated as most studies in the developing world are only performed using microscopy examination. In this work, most participants with Cryptosporidium infection (21/34, 61.8%) were asymptomatic at the time of sampling. Cryptosporidium infections are mainly asymptomatic and self-limited but in young and immunosuppressed individuals it can cause acute watery diarrhea and it has been also associated with colon cancer [61,62]. Moreover, colon cancer patients were found positive for Cryptosporidium sp. using microscopy (32.5%), ELISA (42.5%) and PCR (47.5%) showing significantly higher risk for infection when compared to the control group [62]. Long-term sequelae have been also described in immunocompetent individuals depending on the immune and nutritional status of hosts and parasite species, adaptation and virulence [63]. Several outbreaks have been documented in children [60] and immunocompromised patients, moreover an outbreak affecting also immunocompetent adults was reported in French Guiana and linked to tap water consumption [64]. C. hominis is an etiological agent in outbreaks being clinically more severe and ease of transmission, particularly, higher virulence has been attributed to subtype IbA10G2 [63,64].
The potential pathogen D. fragilis was only found in one case using trichrome stain which is not a microscopy routine test. D. fragilis detection by several stain methods is challenging and little is known about its impact in low-and middle-income countries. Studies in Latin America have shown prevalences up to 40% depending on the region and population group [66]. For instance, D. fragilis has been found in 15% of asymptomatic individuals [55] and 10.3% in children from Brazil [66] and 40.4% in a rural community in Venezuela [56].
Regarding the second most common commensal, E. nana, our results agree with the overall in healthy population (13.9%) [67] although is lower than the reported by Ospina et al. in a young population 78.8% [32]. E. nana is considered an indicator of fecal contamination in food and water, and it has been found in banknotes. Although no pathogenic associations are known its role as modulator of the immune response has not been rule out [67].
None of the cysts presumptively identified as E. histolytica/E.dispar/E. moshkovskii by microscopy were E. histolytica, this is similar to other studies in Colombia and other countries using PCR to detect E. histolytica and reporting very little or no detection of the pathogen (0%-0.39%) [37,57,58,68]. Worldwide trends reveal a decline in Entamoeba infections although a high burden is still seen in some age groups and low-income regions [69]. Prevalence of E. histolytica may vary in the most vulnerable populations however, studies only based on microscopy examination often result in overestimation of this protozoan. A main advantage of molecular detection is to distinguish E. histolytica from commensal species in the Entamoeba complex allowing a better understanding of its epidemiology. The commensal amoeba E. dispar seems to be more prevalent than E. histolytica with 12% prevalence worldwide [69], likewise in this study, we found E. dispar rather than E. histolytica. Although E. dispar has been considered a noninvasive amoeba, studies by Vilela et al. have shown that virulence factors may be selectively expressed in South American strains leading to clinical disease [70].
Polyparasitism has been associated with higher exposure to contaminated environment, high level of environmental contamination and multiple routes of transmission. Here, one third of the parasitized participants had more than one parasite, this is slightly lower than the previously reported in a similar population in Colombia (41.7%) but with similar rate of more than two infections (14% vs 17.4%) [32]. Multiple parasite infections may indicate alterations on the immune response or nutritional status and increased susceptibility to re-infection, severe disease and other host infections. Overall, student young population showed lower level of polyparasitism (37.5%) compared to school children in Colombia (83%) [65] and other vulnerable groups (52-61%) [36,40]. We found that pet owners were protected from polyparasitism, which has been attributed to activation of the immune response.
Pathogens of public health concern such as Cryptosporidium spp., Giardia spp., hookworm and T. gondii were found in pets, moreover Cryptosporidium spp. and Giardia spp. were found in both humans and pets. Furthermore, the estimated prevalence of Cryptosporidium spp. in people with animal contact was 18%, similar to that of those living in non-urban areas [11]. Remarkably, in this survey Cryptosporidium spp. was also highly prevalent in companion animals, this agrees with estimates suggesting that 8% of Cryptosporidium infections are transmitted by pet contact [52]. Studies in companion animals have shown that C. canis and C. felis are frequently found in dogs and cats, whereas C. parvum, the most common species in humans, has been found in a wide range of hosts [50]. C hominis and C. parvum are the most predominant in humans followed by C. canis and C. felis which are host specific species [52,60]. We identified C. felis in one participant who owned three pets, all of them positive for Cryptosporidium spp. This finding of a host specific Cryptosporidium spp. may support transmission between owners and their companion animals. One participant with C. parvum owned two pets but fecal samples from those pets were not submitted for testing.
The prevalence of Cryptosporidium spp. in animal surveys is also highly variable and depends on the geographical area, climate, and environmental contamination. Animals acquire parasites from exposure to untreated water, raw meat feeding or environmental contamination with oocysts [71][72][73]. We reported Cryptosporidium spp. in half of the dogs and cats. Other studies in Latin America have shown the same trend, however the infection rate in our study was substantially higher compared to studies using different detection methods and ranging from 4% to 24.5% [71,74,75]. A recent review in cats reported a worldwide prevalence of Cryptosporidium sp. of 6% [76] whereas in Brazil and Colombia the prevalence in cats was 11.1% and 13% respectively [74,77].
Interestingly, we found T. gondii oocysts in one domestic cat (6.6%). T. gondii is a zoonotic protozoa and its prevalence in humans has been correlated to oocyst contamination of environments and infected stray cats. Several studies in Colombia have shown prevalence of oocyst shedding in cats ranging from 0 to 66% [78][79][80] with fluctuations depending on the region, cat population and low or sporadic oocyst shedding. A recent study using DNA detection by PCR found 17.8% T. gondii positive fecal samples from cats [79]. Oocyst shedding is considered of epidemiological significance for the dynamics and toxoplasmosis transmission in animals and humans, this highlights the need to further investigate T. gondii infections in domestic cats from our region to control the spread.
The proximity among animals and humans and particularly pets in children and young adults represents a potential risk for infection. We observed that several animals in a household are highly likely to be infected with the same parasites suggesting either high transmission and/or environmental contamination. Several factors related to the human and animal habits, the parasite and the environmental conditions may contribute to the high rate of Cryptosporidium spp. infections seen in this survey. Studies in domestic animals have reported a prevalence ranging from 6.7% to 41.6% [76,81], a recent study has reported a pool prevalence of 18% in Latin America [82], 20.3% in pets and 19.9% in livestock, the latter being considered so far as the main parasite reservoir [82]. In Colombia, the Cryptosporidium prevalence in livestock has been reported to be from 13 to 26.6% [83,84].
Overall, the higher susceptibility of young adults to protozoan parasites and particularly Cryptosporidium spp. may be associated with several routes of transmission however, this study points out a relevant level of environmental contamination and the potential role of pets as source of infection. For a One Health approach, it is paramount to monitor the potential sources of contamination and their contribution to human infection; this includes assessment of drinking water and animal health. Previous studies in Latin America have found Cryptosporidium spp. in raw and drinking water [85][86][87][88] as well as outbreaks affecting immunocompetent and immunocompromised individuals [64]. Interaction of humans and animals has been growing and becoming closer in recent years increasing the potential for zoonotic transmission, and spread of pathogens such as Cryptosporidium spp. and G. intestinalis. As suggested by others, surveillance requires molecular detection to understand the real epidemiology as microscopy has poor sensitivity and underestimates the real prevalence of infection [89]. Further characterization of circulating genotypes, virulence and adaptation mechanisms to environmental conditions is needed to understand transmission routes and to focus interventions on human, animal, and environmental health. One limiting factor of this study was the small animal sample, further pet surveys should also assess pet ownership practices, pet habits and behavioural factors.

Conclusions
Prevalence of protozoa in university students was remarkably high suggesting that this group is a relevant susceptible population for surveillance as short-and long-term effects remain to be determined. More attention should be given to young adults as many of them are in school to work transition and underlying morbidity or coinfections may be worsening the outcome of parasitic infections. Particular habits and conditions in this population may increase the exposure to some routes of transmission leading to the selective spread of some parasite types. Molecular subtyping would be useful to determine the main transmission route and the virulence potential or effects of Cryptosporidium spp. and the Blastocystis host interactions.
Control strategies to improve student´s health and to prevent co-infections should be focused on education programs for better identification of contamination sources for both humans and pets and to reinforce food safety to reduce exposure. Boiling of drinking water, well-cooked meals and proper washing of fruit/vegetables should be a basic measurement to control Cryptosporidium spp. as the oocysts are resistant to most disinfection treatments. Our findings also encourage veterinary care and control of parasitic infections in domiciled animals, in addition to implementation of better diagnostics and prophylactic programs in our region. This study has also shown that molecular detection for Cryptosporidium spp. is a need for diagnosis and surveillance.